General Cell Based Assays
Bioassay Systems QuantiChrom™ Magnesium Assay Kit. For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism.
For quantitative determination of Mg2+ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg2+ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM).
Eurofins Discoverx cAMP Hunter eXpress GRM4 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0117E2CP24M)
The cAMP Hunter GRM4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. L-glutamate) based activation of GRM4 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GRM4, a naturally Gi/Go coupled GPCR. Upon activation of GRM4, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Triglyceride Standard
Triglyceride Standard
The product is subject to the following: Abcam Restricted Use Statement
Bioassay Systems EnzyChrom™ AF HDL and LDL/VLDL Assay Kit. For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. Method: OD570nm, or FL530/585nm. Samples: Serum. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.2 mg/dL.
Abcam Free Fatty Acid Assay Kit -¿Quantification
Free Fatty Acid Assay Kit -¿Quantification
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter GLP2R Bioassay Kit, 2-Plate. (93-0572Y2-00133)
The PathHunter GLP2R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. GLP2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress MCHR1 Total Internalization Assay, 10pl Kit. (93-0947E3CP5L)
The PathHunter MCHR1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of MCHR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged MCHR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MCHR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc Lipid Extraction Kit (Chloroform-Free), 50 preps
Traditional lipid extraction by the Folch method is tedious and uses toxic chloroform as the key solvent. In addition, the extracted lipids end up in the bottom layer which is inconvenient. Cell Biolabs' Lipid Extraction Kit (Chloroform-Free) uses an organic solvent system that results in the organic phase being on the top, making it easier to use while eliminating the need for chloroform. Each kit provides sufficient reagents for 50 preps based on a 100µL sample size.
Cayman Chemical Estradiol 3-(β-D-Glucuronide) (sodium salt), Purity: ≥95%, CAS: 14982-12-8, 5 mg
A non-cholestatic regioisomer of E217G that acts as a substrate for MRP2, competing with E217G for MRP2-mediated transport (IC50 = 14.2 µM)
Meso Scale Discovery R-PLEX Human hCG alpha ASY
The R-PLEX Human HCG Alpha Assay Kit from Meso Scale Diagnostics is an electrochemiluminescent immunoassay for measuring human chorionic gonadotropin alpha subunit (hCG ) in various biological matrices It uses MSD s SULFO-TAG labels and multi-array plate technology for sensitive detection The kit includes a 96-well plate with capture antibody detection antibody with ruthenium label calibrators and diluent Sensitivity is 0 05 mIU/mL with an upper limit of 1000 mIU/mL suitable for monitoring hCG levels and pregnancy testing
Electron Microscopy Sciences Flim Sleeve 135 mm/24 exp with Frosted Open Flap
Polyguard Polypropylene film sleeves are the finest archival quality material, and are guaranteed to give you ultimate protection for your most important film negatives. Polyguard is 100% acid free, as well as being free from optical distortion. It is resistant to water, grease, oil, alkalies, and organic solvents. Polyguard is durable, yet pliable and gentle to the film and will not become brittle, crack, or abrade the film.
Type: Frosted Open Flap
Indigo Biosciences Human Granulocyte-Macrophage Colony-Stimulating Factor Receptor (GM-CSFR, CSF2R) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Granulocyte-Macrophage Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against GM-CSFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results
STEMCELL Technologies ALDHbr Assay Kit, For labeling: 6 x 10^7 cells
The ALDHbr Assay Kit is optimized for the identification and quantitation of CD34+ cells that express high levels of the enzyme aldehyde dehydrogenase (ALDH) in human cord blood samples. This kit contains both the CD34+ Cell Detection Kit and the ALDEFLUOR™ Kit. The CD34+ Cell Detection Kit contains pre-selected fluorochrome-conjugated monoclonal antibodies that have been optimized for use with the ALDEFLUOR™ Kit to detect cells that express CD34 and have high ALDH activity by flow cytometry. The CD34+ Cell Detection Kit contains anti-human antibodies APC CD34, PE CD45, and PE-Cyanine5 Glycophorin A/B, and 7-AAD viability dye.A flow cytometer equipped with a 488 nm laser and a 635 nm laser for excitation and appropriate filters for detecting APC, PE, PE-Cyanine5 and 7-AAD fluorescence is required for use.
Cell Biolabs Inc Cellular Senescence Detection Kit (SA-Beta-Gal Staining), 50 assays
Senescent cells show common biochemical markers such as expression of an acidic senescence-associated ß-galactosidase (SA-ß-Gal) activity. While senescence has been characterized primarily in cultured cells, there is also evidence that it occurs in vivo. Cells expressing markers of senescence such as SA-ß-Gal have been identified in normal tissues. Cell Biolabs' Cellular Senescence Assay Kit provides an easy-to-use and efficient method to determine cellular senescence. SA-β-galactosidase catalyzes the hydrolysis of X-gal, which produces a blue color. Each kit provides sufficient quantities to perform up to 50 assays in 35 mm wells.
Cell Biolabs Inc Free Fatty Acid Assay Kit (Colorimetric), 100 assays
Free fatty acids (FFA) are formed from the hydrolyisis of triglyceride ester bonds and serve as a ready energy source by binding to albumin in the bloodstream. Measurement of FFA has become an important part of monitoring various disease states including obesity, diabetes, and cancer. Cell Biolabs' Free Fatty Acid Assay Kit quantifies the FFA concentration (non-esterified) in various samples using a 96-well colorimetric plate format. It can detect FFA levels as low as 15 µM in plasma or serum samples. Each kit provides sufficient reagents for 100 assays including standards and unknown samples.